Capping B (10 % N-methylimidazole in THF / pyridine, V / V = 80 : 10) NC-0803 emp Biotech GmbH

For each synthesis cycle, up to 1 to 2 % of free 5′-hydroxy groups remain after the phosphoramidite coupling step has been completed. By running a subsequent “Capping” step using an anhydride, these free hydroxyl groups are converted to acetates and are hindered from further chain elongation and formation of long oligonucleotides with incorrect sequences. For optimal acetylation, a solution of acetic anhydride in Tetrahydrofuran or acetonitrile (Capping A) will be mixed in situ during reaction with a catalytic acting solution of N-methylimidazole (Capping B). Additives such as pyridine and lutidine function as mild bases to enhance the efficiency of the capping reaction.

Prepacked in
450mL, 28-400 thread glass bottle, emp Biotech part no. NC-0803-M450.0-001
2.5 L with GL45 thread glass bottle, emp Biotech part no. NC-0803-N002.5-001

Product of emp Biotech